Oral bioavailability basically depends on the permeability of the compound at the site of absorption, the metabolic effect it undergoes at the intestinal and hepatic level and also on the intestinal microbiota, among others. The processes that a bioactive compound undergoes from its oral administration to its elimination from the body consist of: the liberation of the compound of interest from the matrix that contains it, from absorption through cell membranes to systemic circulation and distribution in tissues and organs. During this process, the compounds are metabolized as a consequence of their interaction with the enzymes present in the organs they pass through. Finally, they are excreted from the body.
There is evidence that the biological properties of polyphenols depend on their bioavailability and the amount consumed. The bioavailability of phenolic compounds has been determined by the concentration in urine and plasma samples after ingestion of pure compounds or food with known concentrations.
- To assess the effect of the matrix of compounds on bioavailability.
- Study intestinal absorption using the mouse one-step intestinal perfusion model.
- To assess the effect of the intestinal microbiota on intestinal permeability and metabolic profile.
- Identification and quantification of metabolites thanks to the combined use of two chromatographic techniques LC-ESI-LTQ-Orbitrap and HPLC-ESI-MS/MS.